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install IDM on your system the register all exe file and click OK
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select File menu > Tools > Create archive
In the window that appears, select Create archive from existing files
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[Methods of assessing the significance of non-specific modifications of the main cellular plasma membranes components].
Modifications of cellular membranes caused by a variety of toxins and chemical agents are presented in this article. The examination of non-specific modifications may be necessary for recognition of the cells sensitivity to a large number of damaging factors. Non-specific plasma membrane permeability changes are detected by adding calcein AM to cells. This is performed in 1% of sodium acetate, pH 5.5. The identification of various methods of estimating changes in cell membrane permeability is given. These include: direct measurement of calcein-release into the extracellular medium, fluorescence of calcein loaded to cells, determination of the fluorescent cell profile, and estimation of the activity of intracellular esterases. The application 0b46394aab
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